Vol. 9, Special Issue 3 (2024)

Author(s): Amrutha TA and AK Beena

Abstract: This study intended to disclose the microbial tapestry of a randomly collected household dahi sample that exhibited ropiness. Pour plating in Nutrient agar and MRS agar revealed the prevalence of four types of colonies with distinct colony morphology. Preliminary characterisation indicated that none of them belonged to lactic acid bacteria. Molecular level identification confirmed the isolates as Pichia kudriavzevii strain DMA06, Saccharomyces cerevisiae strain DMA05, Staphylococcus warneri strain DMA04 and Staphylococcus saprophyticus strain DMA03. Both Staphylococcal strains were lipolytic and hemolytic but coagulase negative and failed to liquify gelatin. Remarkable proteolytic activity was evident for Saccharomyces cerevisiae and Staphylococcus saprophyticus. Isolates except Pichia kudriavzevii DMA06 showed growth both at 70 °C and 450 °C. It was also observed that none of the isolates were completely inhibited by 6.5 % NaCl. Prevalence of resistance to B-lactams in staphylococci can be a safety concern.

DOI: 10.22271/veterinary.2024.v9.i3Sb.1409