Vol. 9, Special Issue 2 (2024)

Author(s): Monika Bhardwaj, Bhoj Raj Singh, DK Sinha, Sandeep Kumar and K Karthik

Abstract: Canine infectious tracheobronchitis (kennel cough) has worldwide distribution and is one of the most prevalent respiratory infections of dogs causing mild to severe pulmonary diseases in combination with other infectious agents. In the present study, sero-prevalence of Bordetella bronchiseptica was calculated on the basis of micro-agglutination test (MAT), whole cell antigen ELISA (wcELISA) and precipitated protein antigen ELISA (ppELISA). Total 136 serum samples were collected from dogs of different breed, age groups and both sexes from different geographical areas of India (Ahmedabad, Amroha, Chennai, Delhi, Lucknow, Meerut, Mumbai, Palampur, Bareilly). Out of 136 serum samples, 38 (27.9%), 69 (50.7%), 132 (97.1%) were detected positive with MAT, wcELISA and ppELISA, respectively, for the presence of B. bronchiseptica antibodies. Although health status, age and breed had no association with detection of Bordetella antibodies, yet females appeared to be significantly (p, 0.033) more prone for Bordetella bronchiseptica infection. It was found that MAT was the better test than wcELISA and ppELISA in terms of the specificity required for diagnosis. Detection of Bordetella antibodies with wcELISA and ppELISA had no significant correlation with clinical sickness. It is concluded that MAT is better test for serodiagnosis of Bordetella bronchiseptica infection than ELISA.