Vol. 9, Special Issue 2 (2024)

Methods: Fifty Sirohi goats in total, grouped into four age groups: 3-6 months (n = 12), 6-9 months (n = 12), 9-12 months (n = 12), and above 12 months (n = 14). Estimates were made for body weight and body measurements such as body length (inches), wither height (inches), heart girth (inches), and paunch girth (inches). Genomic DNA was extracted from whole blood using the method described by John et al. (1991) followed by PCR amplification. PstI restriction enzymes were used to perform RFLP on the PCR amplicon in order to detect polymorphism.

Results: The studied samples were monomorphic with TT genotype an observed genotypic frequency of TT genotype was 1, while no genotypic frequency was found for the TC and CC genotypes. T allele allelic frequency was 1, whereas C allele allelic frequency was 0. The existence of monomorphism in the populations of Sirohi goats indicates that the T allele has been fixed in the population, which has led to HW disequilibrium. The fact that the POU1F1 gene is monomorphic in our study suggests that there hasn't been a mutation in the gene; hence we were unable to conduct any association study.