Vol. 8, Special Issue 5 (2023)

The most useful tool in assisted reproduction is called "sperm sorting," which divides sperm cells into two groups based on which chromosomes are X (female) or Y (male). This process permits the selection of "Healthy" sperm and the purpose of particular traits, like sex. Dr. Pinkel also developed the first flow cytometer. The sperm sorting technology known as the Beltsville sperm sexing technology was patented by the USDA in April of 1991. Advanced cell sorting devices called flow cytometers employ a laser to stimulate fluorescent dye that attaches to spermatozoa's DNA. Limitation of sex sorting technology includes the cost of equipment and maintenance etc. The area of sexed semen is to increase pregnancy rates. The usage of sexed semen for the generation of female calves is beneficial because the demand for milk production is rising everyday. This technology is a good technique which is largely required at the field level for the production of desired offspring and for sexed semen to be adopted nationally, farmers must receive financial assistance and education.