Vol. 9, Issue 3, Part F (2024)

Author(s): Koushik Das and Kunal M Gawai

Abstract: Lactobacillus helveticus (V3) culture was co-encapsulated by extrusion and emulsion method with L-ascorbic acid (AA) at different concentrations (60, 80, 100, 120 mg/ml) and evaluated for several parameters. 100 mg of AA showed significantly higher cell viability than other treatments. For extrusion method, it was 9.76±0.039 to 10±0.21 log cfu/g and for emulsion method it was 10.13±0.075 to 10.23±0.013 log cfu/g. Beads from emulsion method showed a higher retention (p<0.05) of ascorbic acid (13.47 mg) than extrusion method (12.65 mg) while beads from emulsion method showed higher (p<0.05) probiotic encapsulation efficiency (EE) % (90.64%) than extrusion method (86.31%) and 100 mg AA addition rate gave significantly good results. In case of emulsion method, EE % of AA (73.93%) was higher than extrusion method (69.61%). Beads from emulsion method had a significantly higher (p<0.05) yield (68.32%) than extrusion method (55.21%) and an increased yield was in proportional with the rate of addition of ascorbic acid. Conclusively from the comparison of the above parameters from extrusion and emulsion method addition rate of 100 mg ascorbic acid/100 ml of coating material, showed optimum results.