Vol. 9, Issue 3, Part D (2024)

Author(s): Shaik Neha Zaben, Kancharla Jyothi, Konjeti Anusha and Mullinti Raghunath

Abstract: The present study was carried out to evaluate the efficacy of proteolytic enzymes therapy in postpartum endometritic buffaloes on recovery in terms of PMN cell percentage and color reaction developed by cervical mucus discharge to white side test. A total of 30 pluriparous buffaloes with signs of clinical endometritis were randomly divided into three groups of ten animals each. The buffaloes of Group I were administered intra uterine with a single dose of proteolytic enzymes viz. trypsin, chymotrypsin and papain. Group II buffaloes were treated with ceftiofur sodium intramuscularly and Group III animals served as controls. The cervical mucus was collected from the buffaloes of all groups before and after treatment, and subjected to white side test and endometrial cytology. Before treatment all experimental animals showed positive color reaction to white side test. Whereas, after treatment, 90, 70 and 40 percent of buffaloes were shown negative color reaction to white side test in Group I, Group II and Group III buffaloes, respectively showing highest recovery in Group I treated with proteolytic enzymes. The mean PMN cells percentage before the treatment was 59.3±1.85, 58.9±1.67 and 57.6±2.04, and after the treatment was 6.7±1.13, 21.2±1.35 and 53.8±1.59 in Group I, II and III buffaloes, respectively. The mean PMN percentage revealed significant difference (p<0.05) in Group I and II from pre to post treatment and also revealed significant difference (p<0.05) among groups after treatment. The first service conception rate was highest in Group I (44.44%) followed by Group II (42.85%) and Group III (0%). It can be concluded that intrauterine infusion of proteolytic enzymes declined PMN cells percent in buffaloes with clinical endometritis and might reduce severity of infection.