Vol. 9, Issue 3, Part A (2024)

Author(s): Ningthoukhongjam Linda, Rafiqul Islam, Fazal Ali Ahmed, Sunil Kumar, Dibyajyoti Talukdar, Girin Kalita and Vivek Kumar Gupta

Abstract: In this study, we aimed to conduct a comparative analysis of the impact of various additives, specifically Butylated Hydroxy Toluene (BHT) at different concentrations (0.2, 0.6 and 1 mM), on the quality of Rani boar semen when stored at refrigerated temperature (5 °C). We utilized split samples from 24 ejaculates obtained from four Rani boars, extending them in GEPS extender both with and without inclusion of additives. The semen samples were evaluated at 0, 24, 48 and 72 hours of preservation at 5 °C. Our findings revealed that BHT at a concentration of 1 mM had a significantly positive effect on sperm motility. Furthermore, it resulted in significantly higher sperm viability, membrane integrity and acrosomal integrity (p<0.05), when compared to the other treatment groups, demonstrating a beneficial impact on semen characteristics throughout the 72 hours preservation period. In summary, BHT when administered at a concentration of 1 mM, exhibited promising effects on maintaining boar sperm quality during refrigerated preservation.